teeth staining


Our study has shown that vapour and tobacco heating products cause significantly less staining to teeth than conventional cigarettes.

For the first time at BAT, our scientists assessed and compared a novel vapour product, a tobacco heating product (THP) and a conventional cigarette for their impact on teeth enamel staining. The results were published in the American Journal of Dentistry.


While cigarette smoke caused significant enamel discoloration, the vapour and the aerosol from the THP caused only minimal staining.

It is well known that smoking cigarettes causes stains on teeth that cannot easily be removed by regular brushing, but little is known about such effects from vapour and THPs. So, our scientists conducted in vitro teeth staining studies to compare the effect of a vapour product, our THP glo, and a reference cigarette (3R4F).


Tests were carried out on enamel blocks cut from bovine incisors. To mimic conditions in the mouth, the enamel blocks were first incubated with saliva to allow the formation of a pellicle layer, a protective protein film that normally forms on teeth. The enamel blocks were exposed to the particulate matter (isolated from the smoke/vapour) for 14 days and then whole smoke/vapour (equivalent to one pack of cigarettes per day) for 5 days.

The enamel samples were assessed before, during and after treatment; colour readings were determined by an independent laboratory using an established method involving a commercially available spectrophotometer and trained scientists.

Discoloration of enamel blocks exposed to cigarette smoke was apparent in as little as one day and continued to increase as the concentration of cigarette smoke increased. In contrast, exposure to vapour or THP aerosol resulted in little or no colour change that was comparable to the untreated controls.


"Many studies have postulated that it is the tar in cigarette smoke that stains teeth. We now have a method where we can rapidly assess in the laboratory the level of enamel discoloration by cigarette smoke and vapour from our vapour products and THPs," explains Annette Dalrymple, a Senior Scientist at BAT.

"The data generated from this study clearly shows that the vapour product and THP assessed caused minimal discoloration – very promising for consumers. However, further studies are required to understand the long-term effect on teeth staining and oral health when smokers switch to using new category products."