It has been suggested that these water-soluble components of cigarette smoke may be present in the bloodstream of smokers, however these findings have yet to be validated.
Aqueous extracts are often applied to in vitro disease models, in particular models of cardiovascular disease, where water-soluble components of cigarette smoke are thought to have effects.
In brief, extracts are generated by bubbling mainstream smoke into a specific volume of cell culture medium or phosphate-buffered saline (PBS) with the help of a constant vacuum) . Both the particulate and gas phase water-soluble chemicals are trapped in the medium. Particulates can be removed by filtering the solution prior to use and can be stored at -20°C for up to 1 month.